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【目的】研究三七总皂甙(PNS)对吗啡戒断大鼠皮质、纹状体神经细胞内[Ca2+]的影响,深入探讨PNS抑制吗啡戒断症状的作用机制。【方法】应用剂量递增法皮下注射盐酸吗啡建立吗啡躯体依赖模型,采用腹腔注射纳洛酮建立催促戒断模型,大鼠在给予吗啡的同时用3种不同剂量PNS进行灌胃,记数大鼠的体重变化和跳跃次数,采用流式细胞术测定PNS对吗啡戒断大鼠皮质、纹状体1神经细胞内[Ca2+]的影响。【结果】(1)PNS呈剂量依赖性地抑制戒断大鼠体重减轻和跳跃的发生。(2)慢性吗啡作用可以使皮质、纹状体神经细胞内[Ca2+]升高;纳洛酮催促戒断可迅速逆转皮质、纹状体神经细胞[Ca2+]异常增高;3种不同剂量PNS可以使戒断大鼠脑内[Ca2+]缓慢升高,且呈剂量依赖性。【结论】PNS对吗啡依赖大鼠催促戒断症状的抑制作用可能与其对中枢[Ca2+]的调节有关。
【Objective】To investigate the effect of panax notoginseng saponins (PNS) on [Ca2+] in cortical and striatum neurons of morphine withdrawal rats and further investigate the mechanism of PNS on morphine withdrawal symptoms. 【Methods】 Morphine somatomorphic model was established by subcutaneous injection of morphine hydrochloride in a dose escalation method. The model of morphine withdrawal was established by intraperitoneal injection of naloxone. Rats were given intragastric administration with 3 different doses of PNS at the same time as morphine. The changes in body weight and number of jumps were determined by flow cytometry to determine the effect of PNS on [Ca2+] in cortices and striatum 1 neurons in morphine withdrawal rats. 【Results】 (1) PNS inhibited the weight loss and jump of abstinent rats in a dose-dependent manner. (2) Chronic morphine can increase [Ca2+] in cortical and striatum nerve cells; naloxone can promptly reverse the abnormal increase of [Ca2+] in cortical and striatum nerve cells; PNS can be used in 3 different doses. The [Ca2+] in the brain of withdrawal rats was slowly increased in a dose-dependent manner. 【Conclusion】 The inhibitory effect of PNS on morphine-dependent rats in urging withdrawal symptoms may be related to its regulation of central [Ca2+].