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建立测定乙肝病毒脱氧核糖核酸（ＨＢＶＤＮＡ）的聚合酶链反应－溴乙锭法（ＰＣＲ－ＥＢ），该方法特异性好，灵敏度为１ｐｇＨＢＶＤＮＡ，已能检出ＨＢＶ敏感动物黑猩猩的的最小感染量。应用ＰＣＲ－ＥＢ法测定氯消毒剂对ＨＢＶ的灭活作用表明，有效氯１２５０ｍｇ／Ｌ作用６０分钟或２５００ｍｇ／Ｌ作用３０分钟可使１０倍ＰＣＲ灵敏度的纯化ＨＶＢＤＮＡ转阴；有效氯２５００ｍｇ／Ｌ作用３０分钟以上可使１０倍ＰＣＲ灵敏度的纯化Ｄａｎｅ颗粒中ＨＢＶＤＮＡ转阴；有效氯６２５ｍｇ／Ｌ作用３０分钟或１２５０ｍｇ／Ｌ作用１０分钟可使１０倍ＰＣＲ灵敏度的纯化Ｄａｎｅ颗粒上ＨＢｓＡｇ转阴。因此认为，ＰＣＲ用于评价氯消毒剂对ＨＢＶ的灭活效果是可行的；ＨＢＶＤＮＡ作为ＨＢＶ的灭活指标比ＨＢＶ表面抗原更为直接可靠。
A polymerase chain reaction-ethidium bromide (PCR-EB) method for the determination of hepatitis B virus DNA (HBVDNA) was established. The method is specific and sensitive with a sensitivity of 1 pg HIBVDNA and has been able to detect minimal infection of chimpanzee in HBV-sensitive animals. PCR-EB method was used to determine the effect of chlorine disinfectant on HBV inactivation. The results showed that the effect of 1250 mg / L of available chlorine for 60 minutes or 2500 mg / L for 30 minutes could turn negative 10% of PCR-purified purified HVBDNA; More than 30 minutes of 10-fold PCR sensitivity of purified Dane particles HBVDNA negative; available chlorine 625mg / L for 30 minutes or 1250mg / L for 10 minutes can make 10 times PCR sensitivity of purified Dane particles HBsAg overcast. Therefore, it is feasible that PCR is used to evaluate the disinfection effect of chlorine disinfectant on HBV. HBVDNA is more direct and reliable than HBV surface antigen as an indicator of HBV inactivation.