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目的观察益肾调肝汤Ⅱ号方对多囊卵巢综合征(PCOS)模型大鼠卵泡发育、卵巢黄体生成素受体(LHR)表达的影响。方法 24日龄Wistar大鼠进行皮下埋植左炔诺孕酮结合皮下注射人绒毛膜促性腺激素造模,将造模成功60只大鼠随机分为6组,每组10只,未造模10只大鼠为正常组。分组灌服生理盐水、盐酸二甲双胍片、益肾调肝汤Ⅰ号方、益肾调肝汤Ⅱ号方低、中、高剂量15天后,观察动物卵巢重量,光镜观察卵巢卵泡的大小、黄体数;免疫组化分析LHR在卵巢中的表达情况。结果运用益肾调肝汤Ⅱ号方中、高剂量后,大鼠发育卵泡数增加,不成熟的早期发育卵泡减少,黄体数量增加,LHR表达增加。结论益肾调肝汤Ⅱ号方能明显增加大鼠卵巢重量、减少小卵泡数、增加黄体数,可明显增强LHR在卵巢中的表达,有利于卵泡的发育及排卵。
Objective To observe the effects of Yishen Tiaogan Decoction Ⅱ on ovarian follicle development and ovarian luteinizing hormone receptor (LHR) expression in rats with polycystic ovary syndrome (PCOS). Methods 24-day-old Wistar rats were implanted subcutaneously with levonorgestrel and subcutaneously injected human chorionic gonadotropin. Sixty rats were randomly divided into 6 groups with 10 rats in each group, without modeling Ten rats were normal group. Groups were fed with normal saline, metformin hydrochloride tablets, Yishen Tiaogan Decoction No.1 and Yishen Tiaogan Decoction No.2 with low, medium and high doses for 15 days. The ovarian follicles were observed for weight by light microscope. The ovarian follicle size, Immunohistochemical analysis of LHR expression in the ovary. Results Using Yishen Tiaogan Decoction Ⅱ medium and high doses, the number of developing follicles in rats increased, immature early follicles decreased, the number of corpus luteum increased and the expression of LHR increased. Conclusion Yishen Tiaogan Decoction Ⅱ can significantly increase the weight of ovary, reduce the number of small follicles and increase the number of lutein in rats, which can significantly enhance the expression of LHR in ovary, which is good for follicular development and ovulation.