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目的探讨免疫编辑前后细胞因子诱导的杀伤细胞(cytokine-induced killer,CIK)分泌细胞因子水平及细胞杀伤活性的变化。方法采用Ficoll密度梯度法提取脐血单个核细胞,定向诱导成CIK细胞,即免疫编辑前CIK细胞。Transwell小室共培养CIK细胞与人肺癌A549细胞,分离纯化CIK细胞即免疫编辑后CIK细胞。通过ELISA和MTT法分别检测免疫编辑前后CIK细胞分泌细胞因子水平及细胞毒活性的变化。结果CIK细胞与A549细胞共培养后,其分泌细胞因子IFN-γ和TNF-α的含量较共培养前CIK细胞显著下降(P<0.05),IL-2则略有降低。共培养后CIK细胞杀伤活性明显减弱(P<0.05)。结论CIK细胞与人肺癌A549细胞间存在免疫编辑作用,编辑后的CIK细胞分泌细胞因子水平及杀伤活性均下降。
Objective To investigate the changes of cytokine production and cytotoxicity induced by cytokine-induced killer (CIK) before and after immuno-editing. Methods Umbilical cord blood mononuclear cells were extracted by Ficoll density gradient and induced into CIK cells by immunohistochemistry. Transwell chamber co-culture CIK cells and human lung cancer A549 cells, CIK cells were isolated and purified immune-edited CIK cells. The level of secreted cytokines and cytotoxicity of CIK cells before and after immuno-editing were detected by ELISA and MTT respectively. Results After CIK cells were co-cultured with A549 cells, the levels of cytokines IFN-γ and TNF-α were significantly decreased (P <0.05) and IL-2 slightly lower than those of CIK cells before co-culture. The cytotoxic activity of CIK cells was significantly decreased after co-culture (P <0.05). Conclusion CIK cells and human lung adenocarcinoma A549 cells have the function of immune editing. The levels of cytokines secreted by CIK cells and the cytotoxicity of CIK cells decreased.