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初步建立了兔肝素化血浆脂蛋白脂酶(LPL)和肝脂酶(HL)活性的测定方法,两种脂酶活性以水解三油酸甘油酯(TG)生成游离脂肪酸(FFA)的含量表示。测得雄性日本大耳白兔肝素化后15min血浆LPL、HL活性最大,批内差异分别为4.96%、3.26%;正常兔LPL、HL分别为12.15±4.07μmol·L~(-1)FFA/h·ml、17.11±6.80μmol·L~(-1)FFA/h·ml;实验兔喂饲胆固醇12wk形成动脉粥样硬化(AS)时LPL、HL活性分别为7.94±3.36μmol·L~(-1)FFA/h·ml、4.54±1.52μmol·L~(-1)FFA/h·ml,两者均较正常兔明显降低(P<0.005)。提示:在高胆固醇负荷时,LPL、NL活性降低可能促进了AS的形成。
A method for the determination of rabbit heparinized plasma lipoprotein lipase (LPL) and hepatic lipase (HL) activity was initially established. The two lipase activities were expressed as the amount of free fatty acids (FFA) produced by hydrolysis of triolein (TG). . The activity of LPL and HL in plasma of male Japanese big-eared rabbits was measured 15 min after heparinization. The intra-assay differences were 4.96% and 3.26%, respectively. The normal rabbit LPL and HL were 12.15±4.07 μmol·L -1 FFA/ h · ml, 17.11 ± 6.80μmol · L ~ (-1) FFA/h · ml; experimental rabbits fed cholesterol cholesterol for 12 weeks to form atherosclerosis (AS) LPL, HL activity were 7.94 ± 3.36μmol · L ~ ( -1) FFA/h·ml, 4.54 ± 1.52 μmol·L -1 FFA/h·ml, both of which were significantly lower than normal rabbits (P < 0.005). Tip: In the high cholesterol load, LPL, NL activity may promote the formation of AS.