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骨巨细胞瘤(giant cell tumor of bone,GCT)是一种以多核巨细胞和单核基质细胞组成的常见骨肿瘤,在GCT中,多核巨细胞的存在,始终是该肿瘤的典型组织学特征之一.尽管在GCT的众多研究中发现,GCT中的多核巨细胞可能就是肿瘤环境中的破骨细胞[1～3],并可直接引起骨质的溶解[4,5].但一直未能找到一种有效的方法从GCT中提取高纯度的多核巨细胞,以供体外实验研究.1997年我们在完成国家教委博士点基金和霍英东教育基金课题“骨巨细胞瘤病变部位局部骨质吸收机制”的研究中[6,7],先后尝试运用了percoll密度梯离心法和50μm孔经不锈钢网过滤法,从GCT组织中提取多核巨细胞.结果证实,不锈钢网过滤法确为一种简便、经济而高效的分离提纯方法,值得介绍和推广.
Giant cell tumor of bone (GCT) is a common bone tumor composed of multinucleated giant cells and mononuclear stromal cells. The presence of multinucleated giant cells in GCT is always the typical histological feature of this tumor. One of them. Despite numerous studies in GCT, it was found that multinucleated giant cells in GCT may be osteoclasts in the tumor environment [1-3] and can directly cause bone dissolution [4,5]. Can find an effective method to extract high-purity multinucleated giant cells from GCT for in vitro experimental research. In 1997 we completed the National Education Commission Doctoral Fund and Huo Yingdong Education Fund project "Bone giant cell tumor lesion local bone In the study of absorption mechanism [6,7], successive attempts were made to use percoll density ladder centrifugation and 50 μm pores by stainless steel mesh filtration to extract multinucleated giant cells from GCT tissue. The results confirmed that the stainless steel mesh filtration method was indeed a A simple, economic and efficient separation and purification method is worth introducing and promoting.