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目的 :构建表达卡介苗热休克蛋白 6 5 (BCGHSP6 5 )与人HER 2 /neu细胞毒T淋巴细胞 (CTL)表位 (GP2 )构成的融合蛋白 (BCGHSP6 5 GP2 )的重组质粒。方法 :将用PCR方法扩增并回收的BCGHSP6 5 GP2 基因片段与原核表达质粒PET15b重组 ,经过酶谱分析筛选出正向重组质粒 ,DNA序列分析正向重组质粒。结果 :筛选出 3个正向重组质粒。结论 :重组表达质粒PET15b BCGHSP6 5 GP2 的构建是成功的 ,为进一步表达奠定基础
Objective: To construct a recombinant plasmid expressing fusion protein (BCGHSP6 5 GP2) consisting of BCGHSP6 5 and human HER2 / neu cytotoxic T lymphocyte (CTL) epitope (GP2). Methods: The BCGHSP6 5 GP2 gene fragment amplified and recovered by PCR was recombined with the prokaryotic expression plasmid PET15b. The positive recombinant plasmid was screened by zymogram analysis, and the positive recombinant plasmid was analyzed by DNA sequencing. Results: Three positive recombinant plasmids were screened out. Conclusion: The construction of recombinant plasmid PET15b BCGHSP6 5 GP2 was successful and laid the foundation for further expression