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目的:研究黏蛋白1(mucin1,MUC1)在食管癌发生中的作用,寻找食管癌发病的新机制。方法 :采用MUC1-siRNA和CTL-siRNA质粒转染Ec1.71细胞获得MUC1稳定沉默细胞系,分别通过MTT法、软琼脂克隆形成实验和裸小鼠移植瘤模型检测下调MUC1对鳞状上皮细胞食管癌细胞生长、克隆形成能力和体内成瘤能力的影响。结果:建立Ec1.71/MUC1-siRNA-A/B稳定沉默MUC1细胞系和Ec1.71/CTL-siRNA-A/B对照组细胞系各两株。与对照组Ec1.71/CTL-siRNA-A/B细胞系相比,MUC1沉默组Ec1.71/MUC1-siRNA-A/B细胞的生长速度显著减慢,软琼脂克隆数目明显减少,移植瘤的生长速度明显减慢、肿瘤体积更小。结论:MUC1与食管癌的形成密切相关,抑制其表达,可显著降低食管癌细胞的恶性程度。本研究为食管癌的发病机制研究和临床治疗提供了重要参考。
Objective: To study the role of mucin1 (MUC1) in the pathogenesis of esophageal cancer and to find out a new mechanism of esophageal cancer. Methods: The MUC1 stable silencing cell lines were transfected with Ec1.71 cells using MUC1-siRNA and CTL-siRNA plasmids. MTT assay, soft agar colony formation assay and nude mouse xenograft model were used to detect the effect of MUC1 on esophageal squamous cell carcinoma Cancer cell growth, clonogenicity and in vivo tumorigenicity. Results: Two Ec1.71 / MUC1-siRNA-A / B stable silencing MUC1 cell lines and two Ec1.71 / CTL-siRNA-A / B control cell lines were established. The growth rate of Ec1.71 / MUC1-siRNA-A / B cells in MUC1 silencing group was significantly slower than that of Ec1.71 / CTL-siRNA-A / B cell line in control group, the number of soft agar was significantly decreased, The growth rate slowed down significantly, the tumor volume is smaller. Conclusion: MUC1 is closely related to the formation of esophageal cancer and inhibits the expression of MUC1, which can significantly reduce the malignant degree of esophageal cancer cells. This study provides an important reference for the study of pathogenesis and clinical treatment of esophageal cancer.