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胰岛细胞特染技术,近年来来国内外有不少新的改进方法,各有所长、总的来说A、B细胞染色比较稳定、但D细胞染色不够理想,本人曾对胰岛细胞染色作了一点探索,如硝酸银-醛复红-铅苏木素法,不足之处是切片若久存,则银离子易扩散、导致D细胞褪色,有待改进。本法是在Mallory及Heidenhain AzAn法的基础上,根据胰岛细胞化学特性和染色原理予以改良、操作较简便、效果较好,介绍如下: 一、材料和方法:取豚鼠新鲜胰腺、Helly液固定24小时、常规脱水,石腊包埋,切片厚
Islet cell special dye technology, in recent years at home and abroad there are many new ways to improve, each have their own strengths, in general, A, B cells stained relatively stable, but D cell staining is not ideal, I had on the islet cell staining for A little exploration, such as silver nitrate - aldehyde complex red - lead hematoxylin method, the downside is that if the long section of the chip, the silver ions are easy to spread, leading to D cell fading, to be improved. This method is based on the Mallory and Heidenhain AzAn method, based on the islet cell chemistry and staining principles to be improved, the operation is simpler, the effect is better, described as follows: First, materials and methods: Guinea pigs fresh pancreas, Helly fluid fixation 24 Hours, conventional dehydration, embedded paraffin, sliced thick