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以三倍体枇杷为材料,研究了不同消毒方式、MS培养基浓度、植物生长调节剂及浓度配比对茎尖培养及诱导生根的影响。结果表明,初代培养时,选择生长饱满、健壮的顶芽及适宜的消毒方式,外植体剥离长度0.5~0.8 cm,能显著提高茎尖培养的成活率;MS培养基浓度的变化对外植体的褐化没有明显的影响;最适茎尖的启动培养基为MS+1.0 mg·L-16-BA+0.5 mg·L-1 NAA,成活率高达84.8%;最适组培苗生根培养基为1/2MS+0.1 mg·L-1 NAA+0.01 mg·L-1 IAA+0.3%活性炭,生根率达66.7%,每株平均生根2.83条。该研究结果将为三倍体枇杷再生体系的建立及利用转基因技术对三倍体无籽枇杷进行遗传改良奠定基础。
Using triploid loquat as material, the effects of different disinfection methods, MS medium concentration, plant growth regulators and concentration ratio on shoot tip culture and induced rooting were studied. The results showed that the explants stripped 0.5 to 0.8 cm in length during the initial culture when selecting full-fledged, robust top buds and appropriate disinfection methods. The survival rate of shoot tip culture was significantly improved. The changes of MS medium concentration on explants Browning. There was no significant difference in the browning of the optimum culture medium. The optimum culture medium was MS + 1.0 mg · L-16-BA + 0.5 mg · L-1 NAA with the highest survival rate of 84.8% 1 / 2MS + 0.1 mg · L-1 NAA + 0.01 mg · L-1 IAA + 0.3% activated carbon. The rooting rate was 66.7% and the average rooting rate per plant was 2.83. The results of this study will lay the foundation for the establishment of triploid loquat regeneration system and the genetic improvement of triploid loquat using transgenic technology.