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电子染色是透射电子显微镜样品制备小的关键步骤之一。虽然在国外自动电子染色机已经问世,但因其价格昂贵而未能完全普及。因此多数电镜室还是利用蜡盘、小型平皿等进行电子染色。而电子染色的成败关键之一是防止切片的染色污染。特别是超低倍观察用样品制备时,其要求更为严格。为了防止二氧化碳和铅染液接触形成碳酸铅,在染色器具中放置氢氧化钠颗粒或在染色槽内充满氮气等。但这些并不能完全
Electron staining is one of the key steps in the preparation of transmission electron microscopy samples. Although automatic electronic dyeing machine in foreign countries has come out, but because of its expensive and not fully universal. Therefore, most electron microscope room or the use of wax plates, small plates and other electronic staining. One of the keys to the success or failure of electronic staining is to prevent staining of the sections. Especially for ultra-low magnification samples for observation, the requirements are more stringent. In order to prevent the contact of carbon dioxide and lead dye to form lead carbonate, sodium hydroxide particles are placed in the dyeing equipment or filled with nitrogen in the dyeing tank. But these can not be complete