论文部分内容阅读
目的研究卵巢上皮性癌细胞核DNA含量及其与卵巢癌化疗和预后的关系。方法应用自动图像分析系统对 2 5例术前未化疗及 2 0例术前化疗的卵巢癌组织的DNA指数 (DI)和DNA倍体组成进行测量和分析。结果卵巢癌细胞的DNA指数 (HI)在术前未化疗组和术前化疗组中分别为 3 6 5 4 1± 1 30 6 3,2 2 0 2 0± 0 6 0 38,两组比较差异有极显著意义 (P <0 0 0 1 ) ;DNA倍体构成在化疗前后差异也有显著意义 (P <0 0 0 5 ) ,前者峰值体现于 >5C的非整倍体 ,后者主要位于 3C～ 4C的整倍体群中。结论 (1 )反映肿瘤细胞增殖状态的DNA指数和DNA倍体组成可能与卵巢癌的发生发展之间有一定的相关关系 ,他们可能反映了肿瘤细胞对抗肿瘤药物的敏感程度和耐受程度 ,在卵巢癌的病理诊断 ,恶性程度判定 ,疗效评价及生物学行为和预后评估中可能有重要价值。 (2 )卵巢癌术前化疗再结合手术和观测癌细胞DNA含量的变化 ,可能为选择术后化疗方案提供依据 ,为提高卵巢癌患者的 5年生存率 ,成为将来临床上对卵巢癌可能有前景的治疗手段之一。
Objective To study the relationship between DNA content in epithelial ovarian cancer cells and chemotherapy and prognosis of ovarian cancer. Methods The DNA index (DI) and DNA ploidy of 25 cases of preoperative non-chemotherapy and 20 cases of preoperative chemotherapy were measured and analyzed by automatic image analysis system. Results The DNA index (HI) of ovarian cancer cells in the preoperative non-chemotherapy group and the preoperative chemotherapy group were respectively 3656,14063 and 2200240038, which were significantly different between the two groups (P <0.01). The difference of DNA ploidy before and after chemotherapy was also significant (P <0 05). The former peaked at> 5C aneuploidy, while the latter was mainly located in 3C ~ 4C euploid group. Conclusion (1) DNA index and DNA ploidy, which reflect the proliferation status of tumor cells, may have some correlation with the occurrence and development of ovarian cancer. They may reflect the sensitivity and tolerance of tumor cells to antitumor drugs. Ovarian cancer pathological diagnosis, determine the degree of malignancy, efficacy evaluation and biological behavior and prognosis may have important value. (2) Preoperative chemotherapy of ovarian cancer combined with surgery and observation of changes in DNA content of cancer cells may provide a basis for the choice of postoperative chemotherapy, in order to improve the 5-year survival rate of patients with ovarian cancer, a clinical ovarian cancer may have One of the prospects for the treatment.