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目的探讨ＨＩＶＤＮＡ，ＨＩＶＲＮＡ，Ｐ２４Ａｇ与宿主的相互关系及三者间的内在联系。方法收集１３９例抗ＨＩＶ阳性血标本，用固相免疫酶法测Ｐ２４Ａｇ，定量逆转录聚合酶链生物酶法测定ＨＩＶＲＮＡ和巢式聚合酶链法测ＨＩＶＤＮＡ。结果ＨＩＶＲＮＡ阳性数１０８／１３９，ＨＩＶＤＮＡ阳性数１０９／１３９，Ｐ２４Ａｇ阳性数为３４／１３９。进一步分析，同一标本ＨＩＶＤＮＡ，ＨＩＶＲＮＡ均阳性的占８０％，单一阳性为２０％。结论三种ＨＩＶ检测方法的敏感性、一致性与ＨＩＶ毒株变异、个体宿主免疫应答及抗病毒治疗密切相关。多引物多区域同时测ＨＩＶＤＮＡ，ＨＩＶＲＮＡ和抗原，并结合临床进行综合分析，对加强ＨＩＶ感染机理的理解和疗效考核有实际意义
Objective To investigate the relationship between HIVDNA, HIV RNA, P24Ag and host and the relationship among them. Methods 139 anti-HIV positive blood samples were collected, P24Ag was detected by solid phase immunoenzymatic assay, HIV RNA was detected by quantitative reverse transcription polymerase chain reaction and HIVDNA was detected by nested polymerase chain reaction. Results HIV RNA positive number 108/139, HIVDNA positive number 109/139, P24Ag positive number 34/139. Further analysis, the same specimen HIVDNA, HIVRNA were positive accounted for 80%, a single positive was 20%. Conclusion The sensitivity and consistency of the three HIV detection methods are closely related to the variation of HIV strains, the immune response of individual host and antiviral therapy. Multi-primer simultaneous detection of multiple regions of HIVDNA, HIV RNA and antigen, combined with clinical comprehensive analysis of the mechanism to enhance the understanding of HIV infection and efficacy evaluation of practical significance