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目的通过采用高效液相色谱法测定不同加工方法所得金银花药材中绿原酸和木犀草苷的含量,考察不同加工方法对金银花质量的影响。方法绿原酸含量测定采用Agilent Zorbax SB-C18色谱柱(4.6 mm×250mm,5μm),流动相:乙腈-0.4%磷酸(15∶85);流速:1.0 mL·min-1;检测波长:327 nm;柱温:室温。木犀草苷含量测定采用Hypersil Phenyl-2色谱柱(4.6 mm×150 mm,5μm),流动相:乙腈-0.5%冰醋酸,二元梯度洗脱,0~15 min,乙腈10%~20%,15~30 min,乙腈20%,30~40 min,乙腈20%~30%;流速:1.0 mL·min-1;检测波长:350 nm;柱温:40℃。结果不同加工方法对金银花药材中绿原酸和木犀草苷的含量有一定影响。结论烘干法和微波法所得样品质量较好,且药材的外观色泽与药材内在质量之间存在较为密切的关系。
Objective To determine the effects of different processing methods on the quality of honeysuckle by using HPLC to determine the contents of chlorogenic acid and luteolin in the honeysuckle medicinal materials obtained from different processing methods. Methods The determination of chlorogenic acid was performed on an Agilent Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) with mobile phase of acetonitrile-0.4% phosphoric acid (15:85), flow rate of 1.0 mL · min-1 and detection wavelength of 327 nm; column temperature: room temperature. The content of luteolin was determined by Hypersil Phenyl-2 column (4.6 mm × 150 mm, 5 μm) with mobile phase of acetonitrile-0.5% glacial acetic acid, binary gradient elution, 0-15 min, acetonitrile 10-20% 15-30 min, acetonitrile 20%, 30-40 min, acetonitrile 20% -30%, flow rate 1.0 mL · min-1, detection wavelength 350 nm and column temperature 40 ℃. Results Different processing methods have certain influence on the contents of chlorogenic acid and luteolin in honeysuckle. Conclusion The samples obtained by the drying method and the microwave method are of good quality, and there is a close relationship between the appearance color of medicinal materials and the intrinsic quality of medicinal materials.