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目的探讨二烯丙基硫化物(DAS)对百草枯(PQ)中毒大鼠急性肺损伤的影响。方法 100只雄性Wistar大鼠随机分为5组,正常对照组、PQ 70 mg·kg~(-1)模型组、DAS 25,50和100 mg·kg~(-1)治疗组,每组20只。一次性ig给予PQ 70 mg·kg~(-1)制备PQ中毒模型,DAS组于造模前、后30 min ip给予相应剂量药物,正常对照组ip给予等量生理盐水。各组分别于造模后1,3,6和12 h麻醉大鼠,取右肺下叶进行HE染色,光镜下观察肺组织病理损伤程度并评分;右肺上叶进行肺组织一氧化氮(NO)含量检测;左肺进行支气管肺泡灌洗获取肺泡巨噬细胞(AM),培养24 h取上清,分光光度法测定诱导型一氧化氮合酶(i NOS)含量;培养至72 h,采用细胞免疫化学方法检测AM中i NOS蛋白的表达。结果与正常对照组比较,模型组肺泡结构破坏严重,病理评分明显升高(P<0.01),肺组织中NO含量明显升高(P<0.01),AM培养上清液中i NOS含量明显升高(P<0.01),i NOS蛋白表达增加(P<0.05);与模型组比较,DAS 50 mg·kg~(-1)组在造模后3,6和12 h以及DAS 100 mg·kg~(-1)组在造模后1,3,6和12 h肺泡结构损伤显著减轻,病理评分降低(P<0.05);DAS25和50 mg·kg~(-1)组肺组织中NO和i NOS含量及i NOS蛋白表达减少(P<0.05),DAS 100 mg·kg~(-1)组NO含量减少更为明显(P<0.01)。结论 DAS可能通过抑制PQ中毒大鼠肺组织中的氧化损伤因子减轻肺损伤。
Objective To investigate the effects of diallyl sulphide (DAS) on acute lung injury induced by paraquat (PQ) in rats. Methods 100 male Wistar rats were randomly divided into 5 groups: normal control group, PQ 70 mg · kg -1 model group, DAS 25, 50 and 100 mg · kg -1 groups only. One-time ig given PQ 70 mg · kg ~ (-1) preparation of PQ poisoning model, DAS group before and 30 minutes after the administration of the corresponding dose of ip drug, ip normal control group given the same amount of saline. Rats in each group were anesthetized at 1, 3, 6 and 12 h after modeling, HE staining was performed on the lower lobe of the right lung, the degree of pathological damage of the lung tissue was observed under the light microscope and scored. The upper lobe of the right lung was used for the lung tissue nitric oxide (NO) was detected in the left lung. Bronchoalveolar lavage was performed in the left lung to obtain alveolar macrophages (AMs). The supernatant was collected 24 hours after the culture and the content of inducible nitric oxide synthase (iNOS) was measured by spectrophotometry. Immunocytochemistry was used to detect the expression of iNOS protein in AM. Results Compared with the normal control group, the alveolar structure in the model group was severely damaged and the pathological score was significantly increased (P <0.01). The content of NO in the lung tissue was significantly increased (P <0.01), and the content of iNOS in the supernatant of AM was significantly increased (P <0.05). Compared with the model group, DAS 50 mg · kg -1 at 3, 6 and 12 h after modeling and 100 mg · kg -1 of DAS ~ (-1) group was significantly reduced at 1, 3, 6 and 12 h after modeling, and the pathological score was decreased (P <0.05). In the DAS 25 and 50 mg · kg -1 groups, NO and (P <0.05). The decrease of NO in DAS 100 mg · kg -1 group was more obvious (P <0.01). Conclusion DAS may reduce lung injury by inhibiting oxidative damage in the lung tissue of rats with PQ poisoning.