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目的探讨硒对小鼠急性癫痫发作后脑组织氧化损伤的影响。方法选取健康60只小鼠,分为观察组、对照A组和对照B组,各20只。观察组肌内注射20μg/kg亚硒酸钠注射液,对照A组和对照B组均肌内注射等量的0.9%氯化钠溶液,第8天,观察组和对照A组经腹腔注射600万U/kg青霉素,对照B组经腹腔注射等量的0.9%氯化钠溶液;将三组小鼠的水迷宫逃脱时间、脑组织中MDA、GSH-Px活性和小鼠癫痫发作相关指标进行对比。结果三组小鼠出现癫痫的时间、癫痫发作级别、MDA、GSH-Px活性、水迷宫潜逃时间对比,差异有统计学意义(P<0.05)。结论小鼠急性癫痫会导致脑组织氧化损伤,而对小鼠进行补硒可以显著改善MDA含量、GSH-Px活性,减少对脑组织造成的伤害。
Objective To investigate the effect of selenium on oxidative damage of brain tissue after acute seizure in mice. Methods Sixty healthy mice were selected and divided into observation group, control group A and control group B, 20 each. The observation group was intramuscularly injected with 20μg / kg sodium selenite injection, the control group A and B were injected intraperitoneally with the same amount of 0.9% sodium chloride solution. On the 8th day, the observation group and the control group A were injected intraperitoneally 600 Million U / kg penicillin, the control group B by the same amount of 0.9% sodium chloride solution; the three groups of mice water maze escape time, brain tissue MDA, GSH-Px activity and mouse seizures related indicators Compared. Results The epileptic time, seizure level, MDA, GSH-Px activity and water maze abscission time in the three groups were statistically significant (P <0.05). Conclusions Acute epilepsy in mice can lead to oxidative damage of brain tissue. Supplementation of selenium in mice can significantly improve MDA content, GSH-Px activity and reduce damage to brain tissue.