论文部分内容阅读
目的建立高效毛细管电泳法(HPCE)同时测定苍耳类药材中绿原酸、咖啡酸、阿魏酸、异绿原酸A、异绿原酸C、新绿原酸和1,3-O-二咖啡酰奎宁酸含量的方法。方法以50 mmol·L-1硼砂-水(p H 9.54)为缓冲溶液,未涂渍标准熔融石英毛细管(75μm×64.5 cm,有效长度56 cm)为分离通道,运行电压为25 k V,检测波长为326 nm,毛细管温度为25℃,压力进样为25 k Pa×5 s。结果 7种酚酸的浓度与峰面积的线性关系良好(r>0.999 4);加样回收率为99.85%~102.70%。用此法测定了苍耳类药材中7种酚酸的含量,得到满意结果。结论该方法简单、准确,重复性较好,可用于苍耳类药材内在质量的评价和控制。
OBJECTIVE To establish a high performance capillary electrophoresis (HPCE) method for the simultaneous determination of chlorogenic acid, caffeic acid, ferulic acid, isocrochloric acid, isocrochloric acid, chlorogenic acid, chlorogenic acid and 1,3-O- Method of caffeoylquinic acid content. Methods A standard fused silica capillary (75 μm × 64.5 cm, effective length 56 cm) was used as the separation channel with 50 mmol·L-1 borax-water (p H 9.54) as buffer solution and the operating voltage was 25 kV. The wavelength was 326 nm, the capillary temperature was 25 ° C, and the pressure injection was 25 kPa × 5 s. Results The linear relationship between the concentration of 7 kinds of phenolic acids and the peak area was good (r> 0.999 4). The recoveries of samples were 99.85% ~ 102.70%. The method was used to determine the content of seven kinds of phenolic acids in Xanthium medicinal herbs, and satisfactory results were obtained. Conclusion The method is simple, accurate and reproducible. It can be used to evaluate and control the intrinsic quality of Xanthium medicinal materials.