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为探索痢疾杆菌感染是否产生免疫记忆，本文以福氏痢疾杆菌２ａ经口及腹腔免疫小鼠两次（相隔１４ｄ），间隔三个月后再刺激，应用ＢＡ-ＥＬＩＳＰＯＴ法检测了小鼠Ｐｅｙｅｒ（ＰＰ）淋巴结、肠系膜淋巴结（ＭＬＮ）及脾脏（ＳＰＬ）中特异性ＩｇＡ、ＩｇＧ、ＩｇＭ抗体分泌细胞（ＡＳＣ）的变化；并以ＭＴＴ比色法测定了ＣｏｎＡ诱导的淋巴细胞增殖反应；还观察了小肠粘膜组织切片。实验表明：口服途径能诱导ＰＰ中ＡＳＣ及淋转的回忆反应，小肠粘膜可见淋巴滤泡显著增生；而腹腔途径只在ＳＰＬ中诱导出ＡＳＣ及淋转回忆反应。结果提示：痢疾杆菌感染存在免疫记忆反应。
In order to explore whether Shigella dysenteriae infection produces immune memory, we immunized mice with Shigella flexneri 2a twice a day (14 days apart) and restimulated three months later. PP), the changes of specific IgA, IgG and IgM antibody-secreting cells (ASC) in lymph node, mesenteric lymph node (MLN) and spleen (SPL) were measured. ConA-induced lymphocyte proliferation was measured by MTT assay. Small intestinal mucosal tissue sections. Experiments show that: the oral route can induce PP and lymph node lymphatic recall reaction, small intestinal mucosa showed significant proliferation of lymphoid follicles; while the peritoneal approach induced only in the SPL ASC and lymph node recall reaction. The results suggest that there is immune memory reaction in Shigella infection.