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目的探究丹酚酸B(Salvianolic acid B,SalB)对环磷酰胺(cyclophosphamide,CP)所致蚕豆根尖细胞遗传毒性的拮抗作用。方法应用蚕豆根尖细胞微核试验和染色体畸变试验,计算CP(100μg/ml)组、蒸馏水组,SalB处理组(0.1～50μg/ml)及不同处理方法(方法一:同时加入SalB和CP;方法二:先加SalB,后加CP;方法三:先加CP,后加SalB)的微核率(MNR)、有丝分裂指数(MI)和染色体畸变率(CAR)。结果(1)SalB对蚕豆根尖细胞无致突变作用;(2)3种处理方法都能够抑制CP的诱变作用,其中浓度均为50μg/ml时抗突变作用最好;(3)在相同浓度下,3种处理方法相比较差异无统计学意义,其中方法三的MNR、MI和CAR均呈剂量-效应关系。结论在本试验条件下,SalB对蚕豆根尖细胞无致突变作用,并能促进细胞分裂生长;SalB能显著降低由CP诱导的微核率和染色体畸变率;50μg/ml的SalB抗突变活性最强。
Objective To investigate the antagonistic effect of Salvianolic acid B (SalB) on genotoxicity of Vicia faba root tip cells induced by cyclophosphamide (CP). Methods Vicia faba root tip cell micronucleus test and chromosomal aberration test were used to calculate the effect of Sal B and CP on the treatment of CP (100μg / ml), distilled water and SalB (0.1 ~ 50μg / ml) Method two: first add SalB, then add CP; third method: first add CP, then add SalB) MNR, MI and CAR. Results (1) SalB had no mutagenic effect on Vicia faba root tip cells. (2) All three treatments could inhibit the mutagenicity of CP, and the best mutagenicity was achieved at 50μg / ml. (3) Concentration, there was no significant difference among the three treatment methods. Among them, MNR, MI and CAR of method three showed a dose-effect relationship. Conclusions Under the conditions of this study, SalB had no mutagenic effect on Vicia faba root tip cells and promoted cell division and growth. SalB significantly reduced the frequency of micronuclei and chromosome aberration induced by CP. The anti-mutagenic activity of SalB at 50 μg / ml was the highest Strong.