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以甜菜品种淮阴11号为材料。测定了在不同贮藏条件下甜菜块根呼吸强度、呼吸代谢的酶的活性及呼吸抑制剂的作用。试验结果表明,在温度处理15天以后,冻化贮藏的甜菜块根呼吸强度明显高于其它温度处理的甜菜。糖酵解和已糖磷酸途径抑制剂对呼吸强度的抑制百分比随贮藏时间的延长而加强,而三羧酸代谢途径的抑制剂(丙二酸)对呼吸的抑制程度较低且无明显变化。葡萄糖-6-磷酸脱氢酶活性在甜菜贮藏过程中逐渐加强,尤其是冻化贮藏的甜菜,其活性也明显高于琥珀酸脱氢酶活性。以上结果说明甜菜块根贮藏过程中,呼吸代谢既可通过糖酵解-三羧酸途径,也可通过己糖磷酸途径,且后者的作用随贮藏时间的延长而逐渐加强。另外,呼吸末端氧化是通过细胞色素氧化酶和过氧化氢酶,而贮藏后期多酚氧化酶活力则有明显的增强。
To beet varieties Huaiyin 11 as the material. The respiration intensity, respiratory enzyme activity and respiration inhibitor were determined under different storage conditions. The results showed that the respiration intensity of frozen-stored beet tubers was significantly higher than that of other temperature-treated beets after 15 days of temperature treatment. The percent inhibition of respiration intensity by glycolytic and hexose phosphate pathway inhibitors was enhanced with prolongation of storage time, while the inhibitors of tricarboxylic acid metabolism (malonate) had lower and no significant changes in respiration. Glucose-6-phosphate dehydrogenase activity gradually strengthened during the storage of sugar beet, especially the frozen sugar beet storage, its activity was also significantly higher than succinate dehydrogenase activity. The above results indicate that during the storage of beet root, the respiratory metabolism can be enhanced either by the glycolysis-tricarboxylic acid pathway or by the hexose phosphate pathway, and the latter effect gradually increases with storage time. In addition, the end of the respiratory oxidation through the cytochrome oxidase and catalase, and post-storage polyphenol oxidase activity was significantly enhanced.