论文部分内容阅读
卡介苗(BCG)对某些人群保护效果较差,因此可通过在结核病(TB)亚单位菌苗中加入可诱导Th1细胞[分泌白细胞介素2(IL-2)及γ干扰素(IFN-γ]或Th2细胞(分泌IL-4、IL-5及IL-10)应答的佐剂来提高菌苗的效力.作者将不同佐剂加入结核杆菌的短期培养滤液(ST-CF)中制成TB亚单位菌苗,研究不同住剂对免疫应答的影响.作者在ST-CF中分别加入二甲基-双十八烷基溴化铵(DDA)、氢氧化铝[Al(OH)_3]、弗氏不完全性剂(IFA)、RIBI佐剂或Quil-A皂苷制成实验菌苗,分别注入小鼠后足垫,7天后分离局部淋巴结细胞,用逆转
Bacillus Calmette-Guerin (BCG) is less effective in protecting certain populations, so it is possible to prevent TB infection by adding Th1 cells (IL-2 and IFN-γ ] Or Th2 cells (secreting IL-4, IL-5 and IL-10) response adjuvants to improve the efficacy of the vaccine.The author will be different adjuvants added Mycobacterium tuberculosis short-term culture filtrate (ST-CF) made of TB Subunit bacterin and study the influence of different inhalers on immune response.The authors added dimethyl-dioctadecylammonium bromide (DDA) and aluminum hydroxide [Al (OH) 3] to ST-CF, Freund’s incomplete agent (IFA), RIBI adjuvant or Quil-A saponin were injected into the mouse hind footpad, local lymph node cells were isolated after 7 days,