论文部分内容阅读
建立了以人红细胞为ＳＳＡ抗原来源的免疫印迹法（ＲＢＣ－ＩＢＴ）检测ＳＳＡ抗体。此法的敏感性略低于常规的对流免疫电泳法（ＣＩＥ），但可检出部分ＣＩＥ中阴性的ＳＳＡ抗体阳性血清，而且尚可鉴定出ＳＳＡ抗体的两种亚型（５２ＫＤ和６０ＫＤ）。检测了干燥综合症（ＳＳ）、系统性红斑狼疮（ＳＬＥ）及类风湿性关节炎（ＲＡ）等三组病人血清，其中ＳＳ和ＳＬＥ两组在ＳＳＡ抗体亚型的分布上差异显著。抗５２ＫＤＳＳＡ抗体主要分布于ＳＳ组（Ｐ＜０．０５），而抗６０ＫＤＳＳＡ抗体主要见于ＳＬＥ组（Ｐ＜０．０５），ＲＢＣ－ＩＢＴ中，非特异或不明显色带极少见，而ＳＳＡ抗体带清晰、易辨。
A SSB antibody was developed using human erythrocytes as a source of SSA antigen by Western blotting (RBC-IBT). The sensitivity of this method is slightly lower than conventional convective immuno-electrophoresis (CIE), but some of the negative SSA antibody positive sera can be detected in CIE, and two subtypes (52KD and 60KD) of SSA antibodies can still be identified. The serums of patients with Sjogren’s syndrome (SS), systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) were detected. There were significant differences in the distribution of SSA antibody subtypes among SS and SLE patients. Anti-52KDSSA antibodies mainly distributed in SS group (P <0.05), while anti-60KDSSA antibodies mainly in SLE group (P <0.05), RBC-IBT, non-specific or unclear bands are rare, and SSA Antibody with a clear, easy to distinguish.