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目的:建立同时测定儿茶青黛复合膜中儿茶素、表儿茶素、靛蓝和靛玉红4个成分含量的HPLC方法,以控制该制剂的质量。方法:采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,流动相组成为水(A)-甲醇(B),梯度洗脱[0 min,A-B(75∶25);10 min,A-B(70∶30);15 min,A-B(63∶37);20 min,A-B(45∶55);25 min,A-B(40∶60);50 min,AB(40∶60)],流速1.0 mL·min-1,检测波长285 nm,柱温30℃。结果:本方法可在45 min内完成儿茶素、表儿茶素、靛蓝和靛玉红4个成分的分析,且各成分色谱峰之间具有良好的分离度。儿茶素、表儿茶素、靛蓝和靛红的进样量分别在0.1386～1.386μg(r=0.9999),0.02694～0.2694μg(r=0.9997),0.1289～1.289μg(r=0.9997),0.02860～0.2860μg(r=0.9997)范围内与色谱峰面积呈良好的线性关系;加样回收率(n=6)均在95.8%～97.1%之间,RSD均小于2.0%。结论:该方法可用于儿茶青黛复合膜的质量控制。
Objective: To establish an HPLC method for the simultaneous determination of catechin, epicatechin, indigo and indirubin in Camellia cordifolio complex membrane to control the quality of the preparation. Methods: The mobile phase consisted of water (A) -methanol (B), gradient elution [0 min, AB (75:25), 10 min with Agilent ZORBAX SB-C18 (4.6 mm × 250 mm, , AB (70:30); 15 min, AB (63:37); 20 min AB (45:55); 25 min AB (40:60); 50 min AB 1.0 mL · min-1, detection wavelength 285 nm, column temperature 30 ℃. Results: The method can complete the analysis of four components of catechin, epicatechin, indigo and indirubin in 45 min, with good resolution between the chromatographic peaks of each component. The injection rates of catechin, epicatechin, indigo and isatin were respectively 0.1386-1.386 μg (r = 0.9999), 0.02694-0.2694 μg (r = 0.9997), 0.1289-1.289 μg (r = 0.9997), 0.02860 ~ 0.2860μg (r = 0.9997). The average recoveries (n = 6) ranged from 95.8% to 97.1% with RSDs less than 2.0%. Conclusion: This method can be used to control the quality of Camellia sinensis composite membrane.