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目的:以Bcl-2、Bax蛋白及mRNA表达水平为指标,观察甘草与甘遂反药对配伍对大鼠肾脏功能的影响及与甘草比例的关系。方法:大鼠50只,随机分为甘遂组、甘遂配伍甘草1:0.25组、甘遂配伍甘草1:0.5组、甘遂配伍甘草1:1组和正常对照组,各组均ig给予相应药物,给药剂量依次为2、2.5、3、4g/kg,连续给药28天。采用免疫组化法检测大鼠肾脏中Bcl-2及Bax蛋白水平;采用RT-PCR法检测其肾脏中Bcl-2及Bax mRNA水平。结果:各给药组2、2.5、3、4g/kg Bcl-2在蛋白及mRNA表达水平均显著低于正常对照组;各比例甘草配伍甘遂组2.5、3、4g/kg Bcl-2在蛋白及mRNA表达水平显著高于甘隧组4g/kg,且与甘草比例呈正相关。各给药组2、2.5、3、4g/kg Bax在蛋白及mRNA表达水平均显著高于正常对照组;各比例甘草配伍甘隧组2.5、3、4g/kg Bax在蛋白及mRNA表达水平均显著低于甘隧组4g/kg,且与甘草比例呈负相关。结论:长期应用甘遂可加速大鼠肾脏细胞的凋亡,影响肾脏功能。甘草可抑制甘遂对肾脏的损害,且有明显的剂量依赖关系。
OBJECTIVE: To investigate the effects of licorice root and kansui anti-drug on the renal function of rats and its relationship with the proportion of licorice root by using the expression of Bcl-2 and Bax protein and mRNA as indexes. Methods: Fifty rats were randomly divided into three groups: Gansui group, Gansui with licorice 1: 0.25 group, Gansui with licorice 1: 0.5 group, Gansui with licorice 1: 1 group and normal control group, Corresponding drugs, the dosage was followed by 2,2.5,3,4 g / kg, continuous administration of 28 days. Immunohistochemistry was used to detect the protein levels of Bcl-2 and Bax in rat kidneys. The levels of Bcl-2 and Bax mRNA were detected by RT-PCR. Results: The expression levels of Bcl-2 and Bcl-2 protein in each group were significantly lower than those in normal control group Protein and mRNA expression levels were significantly higher than Ganzhao 4g / kg, and the ratio was positively correlated with licorice. The protein and mRNA expression levels of Bax at 2, 2.5, 3 and 4 g / kg in each administration group were significantly higher than those in the normal control group. The protein and mRNA expression levels of 2.5, 3, and 4 g / kg Bax Significantly lower than Ganzhao 4g / kg, and was negatively correlated with the proportion of licorice. Conclusion: Long-term administration of Kansui can accelerate the apoptosis of rat renal cells, affecting renal function. Licorice can inhibit the damage to the kidneys of Kansui, and there is a clear dose-dependent relationship.