论文部分内容阅读
目的了解湖州地区急性腹泻患者副溶血性弧菌感染发生的季节分布、可疑中毒食品范围、耐药状况、优势血清型及毒力基因等特征,揭示其流行与发展趋势的规律。方法对2014年-2016年肠道门诊急性腹泻患者粪便分离的125株副溶血性弧菌进行药敏试验、血清分型以及PCR方法检测耐热直接溶血素基因tdh、耐热直接溶血素相关基因trh。结果副溶血性弧菌感染发生呈现时间季节聚集趋势,以6月-10月为高峰期。药敏结果显示对氨苄西林及头孢一代耐药率达100.0%,头孢二代敏感率达25.0%,其他常用抗菌药物敏感率为100.0%。17个血清型中O3∶K6最多,占59.2%(74/125),其次是O4∶K8,占10.4%(13/125)。毒力基因PCR检测显示88.0%(110/125)的分离株为tdh~+/trh~-,6.40%(8/125)的分离株为tdh~-/trh~-,2株携带tdh~+/trh~+。结论湖州地区副溶血性弧菌感染发生呈明显的时间季节特征,可疑中毒食品涉及范围广,优势血清型为O3∶K6,其次是O4∶K8。菌株对多数抗菌药物高度敏感,多数菌株携带tdh~+/trh~-。
Objective To understand the seasonal distribution of Vibrio parahaemolyticus infection in acute diarrhea patients in Huzhou area, the range of suspicious poisoning food, drug resistance status, dominant serotypes and virulence genes and reveal the regularity of its prevalence and development trend. Methods A total of 125 strains of Vibrio parahaemolyticus isolated from the stools of patients with acute diarrhea in enteric clinic from 2014 to 2016 were tested for susceptibility. Serum typing and PCR were used to detect the expression of heat-resistant direct hemolysin gene tdh, heat-resistant hemolysin-related genes trh. Results The occurrence of Vibrio parahaemolyticus occurred in the season of seasonal aggregation, with the peak from June to October. Drug susceptibility results showed that the drug resistance rate to ampicillin and cephalosporin was 100.0%, the sensitivity rate of second generation to cefurone was 25.0%, and the sensitivity of other commonly used antibiotics was 100.0%. Of the 17 serogroups, O3: K6 accounted for the largest, accounting for 59.2% (74/125), followed by O4: K8 (10.4%, 13/125). Virulence gene PCR showed that the isolates of tdh ~ + / trh ~ - were 88.0% (110/125) and tdh ~ - / trh ~ - of 6.40% (8/125) / trh ~ +. Conclusion The occurrence of Vibrio parahaemolyticus in Huzhou area showed obvious time-season characteristics. The suspicious poisoned food ranged widely, the predominant serotypes were O3: K6, followed by O4: K8. Strains are highly sensitive to most antibacterials and most strains carry tdh ~ + / trh ~ -.