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目的:研究大鼠肠缺血再灌注后肠损伤中是否存在聚腺苷二磷酸核糖聚合酶-1(PARP-1)激活和凋亡诱导因子(AIF)易位,并探索二者在肠缺血再灌注损伤中的作用。方法:通过阻断腹腔干和肠系膜上动脉30min后,恢复血运,制作鼠肠缺血再灌注模型,分为肠缺血再灌注组(I/R),缺血再灌注前15min经静脉给PARP-1抑制剂3-AB组(Drug)和假手术组(Sham)。取肠组织分别作HE染色、聚腺苷二磷酸核糖(PAR)的免疫组化染色,TUNEL方法检测组织细胞凋亡情况,WesternBlot检测凋亡早期信号PARP-1片段p85和凋亡诱导因子(AIF)的表达情况。结果:I/R组肠损伤程度、PAR阳性表达率、细胞凋亡率和凋亡早期信号PARP-1p85片段、AIF表达程度均较Sham组显著增高(P<0.05);应用PARP-1抑制剂后,Drug组上述指标均较I/R组显著降低(P<0.05),但仍高于Sham组。结论:在大鼠肠缺血再灌注损伤中存在PARP-1激活和AIF易位两分子事件,并在肠缺血再灌注损伤中发挥重要作用;肠缺血再灌注前应用PARP-1抑制剂有肠保护效果。
AIM: To investigate whether PARP-1 activation and apoptosis-inducing factor (AIF) translocation are involved in the intestinal injury after intestinal ischemia-reperfusion in rats and to explore the role of PARP- The role of blood reperfusion injury. Methods: After blocking the celiac trunk and superior mesenteric artery for 30 minutes, the model of rat intestinal ischemia / reperfusion was established. The rats were divided into intestinal ischemia reperfusion group (I / R), ischemia 15 min before intravenous injection PARP-1 inhibitor 3-AB group and sham group. The intestinal tissues were harvested for HE staining and immunohistochemical staining of poly (ADP-ribose) (PAR). TUNEL method was used to detect the apoptosis of tissue cells. PARP-1 fragment p85 and apoptosis-inducing factor (AIF ) Of the expression. Results: Compared with Sham group, the expression of PARP-1p85 fragment and AIF in intestinal injury, PAR positive expression rate, apoptosis rate and early apoptotic signal in I / R group were significantly increased (P <0.05). PARP-1 inhibitor Afterwards, the above indexes in the Drug group were significantly lower than those in the I / R group (P <0.05), but still higher than those in the Sham group. CONCLUSION: PARP-1 activation and AIF translocation appear in intestinal ischemia-reperfusion injury in rats and play an important role in intestinal ischemia-reperfusion injury. Pretreatment with PARP-1 inhibitor Intestinal protection effect.