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目的探讨缺氧诱导因子1α(HIF-1α)蛋白、多药耐药相关蛋白1(MRP1)在脊索瘤中的表达水平及其与脊索瘤病理特征和放化疗抵抗之间的关系。方法收集2000年1月-2008年12月接受手术治疗的50例脊索瘤患者的肿瘤组织石蜡标本,另取15例骨软骨瘤标本作为对照。50例脊索瘤患者中男35例,女15例;年龄17～77岁,平均53.3岁;原发病例26例,复发病例24例;病理类型包括普通型48例,去分化型1例,外周型1例。采用免疫组化Envision法检测HIF-1α、MRP1蛋白表达情况,分析其与放化疗抵抗的关系,并探讨HIF-1α、MRP1表达与脊索瘤临床病理特征的关系。结果50例脊索瘤组织HIF-1α、MRP1的阳性表达率分别为80%和74%,均明显高于骨软骨瘤组织(分别为20%和27%,P<0.01)。相关性分析显示,脊索瘤组织中HIF-1α与MRP1的表达呈正相关关系(r=0.80,P<0.01)。脊索瘤组织中HIF-1α、MRP1的表达水平与患者性别、年龄、发病次数及肿瘤大小、部位、分化程度、分期、淋巴结转移均无明显关系。结论脊索瘤组织中HIF-1α和MRP1表达明显增高,且两者表达水平呈正相关,其过度表达可能在脊索瘤的放化疗抵抗中具有重要作用。
Objective To investigate the expression of hypoxia inducible factor 1α (HIF-1α) and multidrug resistance-associated protein 1 (MRP1) in chordoma and its relationship with pathological features of chordomas and chemoradiation and chemoradiotherapy. Methods The paraffin specimens from 50 patients with chordoma admitted to our hospital from January 2000 to December 2008 were collected. Fifteen specimens of osteochondroma were collected as controls. 50 cases of chordoma patients, 35 males and 15 females; aged 17 to 77 years, mean 53.3 years; 26 cases of primary and recurrent cases in 24 cases; pathological types include 48 cases of normal type, dedifferentiation in 1 case, peripheral 1 case. The expression of HIF-1α and MRP1 protein was detected by immunohistochemical Envision method. The relationship between the expression of HIF-1α and MRP1 and chemoradiotherapy and chemosensitivity was analyzed. The relationship between the expression of HIF-1α and MRP1 and clinicopathological features of chordoma was also explored. Results The positive rates of HIF-1α and MRP1 in 50 cases of chordoma were 80% and 74%, respectively, which were significantly higher than those in osteochondroma (20% and 27%, P <0.01, respectively). Correlation analysis showed that there was a positive correlation between the expression of HIF-1α and MRP1 in chordoma (r = 0.80, P <0.01). The expression of HIF-1α and MRP1 in chordoma had no significant correlation with the gender, age, frequency of onset, tumor size, location, degree of differentiation, stage and lymph node metastasis. Conclusions The expressions of HIF-1α and MRP1 in chordoma tissues are significantly increased, and the expression of HIF-1α and MRP1 is positively correlated. The overexpression of HIF-1α and MRP1 may play an important role in chemoradiotherapy of chordoma.