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病毒病是植物的主要病害,利用基因工程防治病毒病已取得巨大进展.成功的主要策略有;①导入病毒的外壳蛋白基因;②利用病毒的复制酶基因,③利用病毒卫星RNA;④利用cRNA或反义RNA技术;⑤利用植物编码的抗病毒基因;③利用抗体如scFV和人体干扰素;⑦利用病毒蛋白.利用病毒外壳蛋白基因是培育抗病毒转基因植物广泛采用的方法之一.已构建了抗烟草花叶病毒(TMV)、首蓿花叶病毒(AMV)、烟草线条病毒(TSV)、马铃薯X病毒(PVX)、马铃薯Y病毒(PVY)、大麦黄矮病毒(BYDV)、黄瓜花叶病毒(CMV)、玉米矮化花叶病毒(MDMV)、水稻条纹叶枯病毒(RSV)等30多种病毒的转基因植物,部分已进入田间试验和生产应用。
Virus disease is a major plant disease, the use of genetic engineering has made tremendous progress in the prevention and treatment of virus disease. The main strategies for success include: (1) introduction of the coat protein gene of the virus; (2) use of the viral replicase gene; (3) use of viral satellite RNA; (4) use of cRNA or antisense RNA technology; (5) use of plant- scFV and human interferon; ⑦ use of viral proteins. The use of the viral coat protein gene is one of the widely used methods of developing antiviral transgenic plants. Tobacco mosaic virus (TMV), first inoculum mosaic virus (AMV), tobacco line virus (TSV), potato virus X (PVX), potato y virus (PVY), yellow barnyard dwarf virus BYDV Cucumber mosaic virus (CMV), maize dwarf mosaic virus (MDMV), rice stripe virus (RSV) more than 30 kinds of virus transgenic plants, some have entered the field trials and production applications.