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在１９８５～１９９５年期间，先后对国内１６个省、市、区的１３９４份玉米青枯病株进行分离，腐霉的分离频率为６３．８％，而镰刀菌则为３６．４％。两菌在各省市区的分离频率不尽相同。深入研究的结果表明，培养基种类、分离部位、不同病级的病株或病株分离（采集）的季节对分离结果有直接影响。根据Ｋｏｃｈ氏法则，从病株上分离的腐霉和镰刀菌必须回接在感病的玉米株上，测定其致病性。接种结果表明，腐霉具有致病性，应是玉米青枯病的病原菌，而镰刀菌则不是。研究结果还表明，接种方式、玉米生育期、接种的菌量、盆土含水量对致病性测定的成败具有重大影响
Between 1985 and 1995, 1,394 strains of corn bacterial wilt were isolated from 16 provinces, cities and districts in China. The separation frequency of Pythium was 63.8% and that of Fusarium was 36.4%. The separation frequency of the two bacteria in various provinces and cities varies. The results of in-depth study showed that the season of isolation and collection of the diseased plants or diseased plants with different types of culture medium, isolated parts and different disease grades had a direct impact on the separation results. According to Koch’s law, Pythium and Fusarium that have been isolated from diseased plants must be rewound on susceptible maize strains and tested for their pathogenicity. Inoculation results show that Pythium is pathogenic, should be the pathogen of corn bacterial wilt, but Fusarium is not. The results also show that inoculation, corn growth period, the amount of bacteria inoculated, basin water content has a significant impact on the success of the pathogenicity test