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以模式生物酵母细胞为材料,研究亚砷酸钠胁迫对细胞死亡率和胞内NO水平的影响,以探讨NO在砷诱导细胞死亡中的作用.结果显示,浓度为1～7mmol·L-1的亚砷酸钠可降低酵母细胞活性,诱导细胞死亡,随着处理浓度的升高和作用时间的延长,细胞死亡率增高;死细胞出现核固缩和核降解等凋亡特征;凋亡抑制剂Z-Asp-CH2-DCB(2“mol·L-1)与3mmol·L-1亚砷酸钠共同作用后,酵母细胞死亡率下降.在亚砷酸钠胁迫的过程中,酵母细胞内NO水平升高;一定浓度的NO清除剂c-PTIO(0.2mmol·L-1)或NO生成抑制剂NaN3(1mmol·L-1)均可降低亚砷酸钠引起的酵母细胞死亡率.结果表明,砷胁迫诱导的胞内NO升高是酵母细胞死亡的一个诱因,亚砷酸钠诱发的酵母细胞死亡中可能存在细胞凋亡过程.
To study the effect of sodium arsenite stress on cell death rate and intracellular NO level in order to explore the role of NO in arsenic-induced cell death, the results showed that the concentration of 1 ~ 7mmol·L-1 Of sodium arsenite can reduce the activity of yeast cells, induce cell death, with the increase of treatment concentration and prolongation of action time, cell death rate increased; apoptotic features such as nuclear condensation and nuclear degradation of dead cells; apoptosis inhibition Z-Asp-CH2-DCB (2 ”mol·L-1) combined with 3 mmol·L-1 sodium arsenite decreased yeast cell death rate in the course of sodium arsenite stress, yeast cells NO concentration increased. A certain concentration of NO scavenger c-PTIO (0.2 mmol·L-1) or NO production inhibitor NaN3 (1 mmol·L-1) decreased the sodium arsenite-induced yeast cell death rate. The results showed that elevated intracellular NO induced by arsenic stress is an inducement of yeast cell death. Apoptosis may occur in yeast cells induced by sodium arsenite.