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香蕉历来是用吸芽繁殖,繁殖速度慢,不能适应当前农业发展和市场的需求。为此,我们以几个香蕉品种为试材,用组织培养技术开展快速繁殖试验。现已成功地诱导出再生植株。供试材料有“中山过山香”、“暹逻过山香”、“牛奶蕉”和“大蕉”等品种的吸芽,经消毒后,在无菌条件下,除去重叠的鞘叶,把芽梢切成顶芽和腋芽两种类型的外植体,接种在含糖3％,并附加苄基氨基嘌呤、呋喃氨基嘌呤和吲哚丁酸等的MS培养基上,置于25—28℃培养室暗培养,经一段时间后其结果: (1)顶芽的外植体经30天培养开始萌动,
Banana has always been to use suction bud propagation, propagation slow, can not adapt to the current agricultural development and market demand. To this end, we use several varieties of banana as test material, with tissue culture technology to carry out rapid propagation test. Regeneration plants have now been successfully induced. The test materials were “Zhongshan over Shannon”, “Siam over Shannon,” “milk banana” and “plantain” and other varieties of suction buds, after disinfection, under sterile conditions, remove the overlap of the leaves, the bud Tops and axillary buds were dissected into two types of explants and inoculated on MS medium containing 3% of sugar and added with benzylaminopurine, furanaminopurine and indole butyric acid, etc., and placed at 25-28 ° C Culture room dark culture, the results after a period of time: (1) top bud explants began to germinate after 30 days of culture,