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作者用rRNA基因探针研究了曼氏血吸虫在未成熟期接触海肯松(hycanthone,HC)后,对HC产生抗性的基因基础。实验采用曼氏血吸虫的JHU和NMRI株。两株均对HC敏感。将二株血吸虫分成若干组。其中一部分组的前代血吸虫用HC处理,以诱导HC抗性。即在感染小鼠28天后给鼠肌注80mg/kgHC碱。而另一些组不用HC处理。肌注HC后的第3～4周解剖小鼠,收集成虫,其虫卵用于感染钉螺,得到的尾蚴再感染小鼠。如此所得的许多代成虫,用于HC抗性测定。即将每代虫感染的小鼠在感染6周后注射80mg/kgHC碱,4周后收集成虫,计算每组每代虫的HC抗
The authors used rRNA gene probes to study the genetic basis of resistance to Schistosoma mansoni in hyManthone (HC) after immature exposure. The experiment used Schistosoma mansoni JHU and NMRI strains. Both strains are sensitive to HC. Two strains of schistosomiasis will be divided into several groups. Some of these groups of pre-passage schistosomes were treated with HC to induce HC resistance. That is, mice were intramuscularly injected with 80 mg / kg ofHC base 28 days after the infection of the mice. While others are not treated with HC. The mice were dissected from 3 to 4 weeks after intramuscular injection of HC and the adults were collected. The eggs were used to infect snails, and the mice were infected with cercariae. Many generations of adult worms so obtained were used for HC resistance assays. The mice infected with each generation of worms were injected with 80 mg / kg HC alkali after 6 weeks of infection and the adults were harvested after 4 weeks. The HC anti