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细胞周期内核仁形成区(NOR)的银可染性反映了NOR中rDNA的转录活性。银离子对NOR中具有转灵活性的rDNA区域有特异的结合性。银染物质是活性rDNA特异结合的酸性蛋白质。生化和免疫化学研究表明银染蛋白质可能是RNA聚合酶Ⅰ,该酶的功能是特异与有活性的rDNA结合催化转录rENA。人类核仁形成区是28s、18s和5.8s rRNA基因所在的位置。因此,对活性核仁形成区进行银染(Ag-NOR),可简便、快速地显示一细胞内银染蛋白、活性rDNA和rRNA三者之间的关系。近来,已发现Ag-NOR可
The silver dyeability of the nucleolar organizer region (NOR) during the cell cycle reflects the transcriptional activity of rDNA in NOR. Silver ions have a specific binding to the rDNA regions that are transcytologically active in NOR. Silver-stained material is an acidic protein that specifically binds active rDNA. Biochemical and immunochemical studies have shown that silver-stained proteins may be RNA polymerase I, whose function is specific to the active rDNA-binding catalytic transcription of rENA. The human nucleolar region is where the 28s, 18s and 5.8s rRNA genes are located. Therefore, silver-stained (Ag-NOR) of the active nucleolar organizer region shows the relationship between intracellular silver-stained protein, active rDNA and rRNA easily and rapidly. Recently, Ag-NOR has been found