论文部分内容阅读
采用Solixa高通量测序技术对比了沉积物微生物燃料电池(sediment microbial fuel cells,SMFCs)开闭路条件下启动阳极生物膜细菌群落结构差异.SMFCs开路条件下启动阳极生物膜获得优化序列3 936条,经过97%相似度归并后得到1 581个操作分类单元(operational taxonomic units,OTUs);SMFCs连接5 kΩ电阻闭路启动阳极生物膜获得优化序列3 930条,聚类归并后产生1 551个OTUs,α多样性指数分析表明开路条件下启动阳极生物膜菌群多样性相对更丰富.SMFCs开、闭路条件下启动阳极生物膜优势菌均为Proteobacteria、Firmicutes和Bacteroidetes,它们在开路下阳极生物膜中的含量分别为59.79%、12.54%和9.02%,而在闭路条件下分别为63.02%、10.01%和3.60%,SMFCs闭路条件下运行,地杆菌Geobacter在阳极生物膜中含量高达16.55%.SMFCs开、闭路条件下启动阳极生物膜细菌群落结构的差异说明SMFCs启动过程电子的传递影响电极生物膜微生物群落结构.
Solixa high-throughput sequencing was used to compare the bacterial community structures of biofilm biosensors under open-circuit conditions of sediment microbial fuel cells (SMFCs) .According to the open circuit conditions, 3 936 optimized biofilms were obtained, After the integration of 97% similarity, 1,581 operational taxonomic units (OTUs) were obtained. SMFCs connected with 5 kΩ resistance closed-circuit anode biofilm obtained 3 930 optimized sequences and generated 1 551 OTUs after clustering Diversity index analysis showed that under the open-circuit conditions, the biomembrane of starter biofilm was relatively richer.Micobacter, Firmicutes and Bacteroidetes, which were the dominant biofilm starters under open and closed conditions, were all exposed in open anode biofilm Were 59.79%, 12.54% and 9.02%, respectively, while they were 63.02%, 10.01% and 3.60% under closed circuit conditions respectively. Geobacter content in the anode biofilm reached 16.55% Differences in the bacterial community structure of the starting biofilm under the conditions indicated that the electron transfer of the SMFCs during the startup process affected the biofilm Biological community structure.