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目的:建立一种简便灵敏的气相质谱法用于测定丙卡特罗的血药浓度。方法:采用电子轰击离子化气相质谱法,以丙咪嗪做内标,样品采用液-液萃取、衍生化处理,分离柱为毛细管柱,进样器和接口温度分别为280℃和250℃,载气(氦气)流速为0.8 mL·min~(-1),进样口选择脉冲不分流模式,离子源和四极杆的温度分别为230℃和150℃。结果:测定方法的检测限为5 ng·L~(-1);线性范围为10-10000 ng·L~(-1);日内(n=5)和日间(n=5)变异系数均小于10％,平均回收率为99.1％±1.3％。结论:本方法灵敏、简便,可用于丙卡特罗的药代动力学研究。
Objective: To establish a simple and sensitive gas chromatography mass spectrometry for the determination of procaterol blood concentration. Methods: Electron bombardment ionization mass spectrometry (GC-MS) was used with imipramine as internal standard. The samples were subjected to liquid-liquid extraction and derivatization. The separation column was a capillary column. The injector and interface temperatures were 280 ℃ and 250 ℃, respectively. The flow rate of carrier gas (helium) was 0.8 mL · min -1. The inlet was pulsed splitless. The ion source and quadrupole temperature were 230 ℃ and 150 ℃, respectively. Results: The detection limit was 5 ng · L -1. The linear range was 10-10000 ng · L -1. The coefficients of variation (n = 5) and daytime (n = 5) Less than 10%, the average recovery was 99.1% ± 1.3%. Conclusion: The method is sensitive and simple, and can be used to study the pharmacokinetics of procaterol.