原花青素对瘢痕疙瘩成纤维细胞增殖、迁移及TLR4/MyD88通路的影响
[关键词]原花青素;瘢痕疙瘩成纤维细胞;细胞增殖;细胞迁移;TLR4/MyD88通路
[中图分类号]R619+.6 [文献标志码]A [文章编号]1008-6455（2021）11-0085-05
Effect of Procyanidins on the Proliferation，Migration and Tlr4/Myd88 Pathway of Keloid Fibroblasts
YANG Jing，XIE Qun
（Department of Plastic Surgery，Xiangyang Central Hospital，Xiangyang 441000，Hubei，China）
Abstract： Objective To investigate the effect of procyanidins on the proliferation， migration and TLR4/MyD88 pathway of keloid fibroblasts. Methods Culture 96-well （5×103 cells/well） KFs cells in vitro， and determine the half inhibitory concentration of proanthocyanidin by MTT method; 96-well （5×103 cells/well） KFs cells were randomly divided into control group， low-dose proanthocyanidin group， and proanthocyanidin medium The dose group and the high-dose proanthocyanidin group， each group has 24 holes （5×103 pieces/hole）. The control group cells were not treated; the proanthocyanidin low， medium， and high-dose groups were treated with proanthocyanidins at final concentrations of 25， 50， and 100 mg/L respectively to interfere with KFs cells. After 24 hours， MTT method， flow cytometry and cell scratch method were used to detect the proliferation rate， apoptosis rate and migration ability of KFs cells， enzyme-linked immunosorbent assay was used to detect the levels of IL-23， IL-17A and IL-6 in KFs cells. Real-time fluorescent PCR and Western Blot method to detect TLR4， MyD88 and NF-κB mRNA and protein levels in KFs cells. Results As the concentration of proanthocyanidins increases， the proliferation rate of KFs cells decreases， and 50 mg/L is the half inhibitory concentration. Compared with the control group， the cell migration distance of the other groups decreased， and the apoptosis rate increased. The mRNA and protein levels of IL-23， IL-17A， IL-6， TLR4， MyD88， and NF-κB in the cells decreased， and with procyanidins With the increase of the dose， the changes in the indicators of each proanthocyanidin dose group showed a dose-response relationship， and the difference was statistically significant （P
Key words： procyanidins; keloid fibroblasts; cell proliferation; cell migration; TLR4/MyD88 pathway
瘢痕疙瘩是皮肤伤口愈合的病理反应，其特征是成纤维细胞的异常增殖和胶原的过度沉积，临床表现为创面边缘外的持续性肿瘤样增生和邻近组织侵犯[1]。