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大鼠左腓总神经离断后与胫神经端侧吻合，于术后第1至8周取L4～5脊髓和背根节（DRG）做冰冻切片，分别显示前角运动神经元（SMN）AChE活性和DRG神经元NOS活性。结果发现；①正常鼠SMN－AChE活性中－强度阳性反应。实验组外侧核SMN－AChE活性和阳性细胞数，于术后2～3周比正常者明显减低，有的SMN－核周体出现空泡样变性；术后4～8周，SMN酶活性和阳性细胞数渐增，并明显高于正常者。同体对照组与实验组同期比较，SMN酶活性相对较弱。②正常鼠部分中－小型DRG神经元NOS活性为中－强度阳性反应。实验组于术后第1～4周，NOS活性和阳性细胞数较正常者明显增高；术后第5～8周其渐趋正常。对照组DRG神经元NOS活性表达较强及与实验组有相应的变化梯度。二组DRG内均见到空泡样变性的神经元核周体。结果提示，坐骨神经慢性损伤所产生SMN－AChE活性增强，可能是轴突再生的反应，并对由NO介导的一级感觉信息传导有一定影响。
After the left common peroneal nerve was transected, the rats were anastomosed to the tibial nerve. L4 ~ 5 spinal cord and dorsal root ganglion (DRG) were harvested from the first week to the eighth week after operation and frozen sectioned. The anterior horn motor neuron (SMN) AChE Activity and NOS activity of DRG neurons. The results showed that: ① normal rat SMN-AChE activity - the intensity of the positive reaction. The activity of SMN-AChE and the number of positive cells in the lateral nucleus of the experimental group were significantly lower than those in the normal group 2 to 3 weeks after operation, and some SMN-perinuclear bodies showed vacuolar degeneration. After 4 to 8 weeks, SMN activity and Positive cells increased, and significantly higher than normal. Compared with the same period in the control group and the experimental group, SMN enzyme activity was relatively weak. ② The NOS activity of mid- and mini-DRG neurons in normal rats was moderate-intensity positive. The experimental group in the first 4 weeks after surgery, NOS activity and positive cells were significantly higher than normal; 5 to 8 weeks after the surgery became normal. The NOS activity of DRG neurons in the control group was stronger than that in the experimental group. Two groups of DRG are seen vacuolar degeneration of neuron perinuclear body. The results suggest that the increased activity of SMN-AChE induced by chronic sciatic nerve injury may be the response of axonal regeneration and may have some influence on NO-mediated primary sensory information transduction.