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目的：探讨成纤维细胞是否为晚期糖基化终产物修饰的β２微球蛋白（ＡＧＥ－β２Ｍ）生物学效应的靶细胞。方法：放射性配体结合实验；［３Ｈ］－ＴｄＲ试验和直接密度计数。结果：［１２５］Ｉ－ＡＧＥ－β２Ｍ能以特异、剂量依赖的方式与人成纤维细胞（ＨＳＦ）结合，这一过程可被抗ＲＡＧＥＩｇＧ所抑制。ＡＧＥ－β２Ｍ能刺激ＨＳＦｓ的增殖，该反应可被抗ＲＡＧＥＩｇＧ、但不被抗ＥＧＦ中和抗体所抑制。结论：ＡＧＥ－β２Ｍ可能通过与成纤维细胞ＲＡＧＥ的相互作用参与透析相关性淀粉样变的发生。
AIM: To investigate whether fibroblasts are target cells for biological effects of advanced glycation end products modified β2-microglobulin (AGE-β2M). Methods: Radioligand binding assay; [3H] -TdR assay and direct densitometry. RESULTS: I-AGE-β2M binds to human fibroblasts (HSF) in a specific, dose-dependent manner, and this process can be inhibited by anti-RAGEIgG. AGE-β2M stimulates the proliferation of HSFs, which is resistant to RAGEIgG but not to anti-EGF neutralizing antibodies. Conclusion: AGE-β2M may participate in the development of dialysis-related amyloidosis through the interaction with fibroblast RAGE.