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目的观察一氧化氮供体DNP对人鼻咽癌细胞株CNE细胞增殖和迁移的影响,并初步探讨其作用机制。方法体外传代培养CNE细胞,按对数浓度差0.1的间距加入0.01～10μmol.L-1DNP,作用48 h后,噻唑蓝(MTT)比色法检测DNP对CNE细胞增殖的影响;Transwell小室法测定细胞的迁移情况;硝酸酶法检测细胞培养液中一氧化氮(NO)的含量;RT-PCR检测CNE细胞中血管内皮生长因子(VEGF)的表达。结果 DNP作用于CNE 48 h后,与空白组比较,CNE的增殖和迁移均受到不同程度的抑制(P<0.05),VEGF mRNA的表达下调,细胞培养液中的NO含量明显提高。结论 DNP能够抑制CNE的增殖和迁移,其机制可能与增加NO含量、抑制VEGF mRNA的表达有关。
Objective To observe the effects of nitric oxide donor DNP on the proliferation and migration of human nasopharyngeal carcinoma cell line CNE and to explore its mechanism. Methods CNE cells were subcultured in vitro, 0.01 ~ 10μmol.L-1 DNP was added at a logarithmic concentration difference of 0.1, and the effect of DNP on the proliferation of CNE cells was detected by MTT assay after 48 h. The migration of cells was detected by enzyme-linked immunosorbent assay (ELISA). Nitric oxide was used to detect the content of nitric oxide (NO) in cell culture medium. The expression of vascular endothelial growth factor (VEGF) in CNE cells was detected by RT-PCR. Results Compared with the blank group, the proliferation and migration of CNE were inhibited by DNP for 48 h (P <0.05). The expression of VEGF mRNA was down-regulated and the content of NO in cell culture medium was significantly increased. Conclusion DNP can inhibit the proliferation and migration of CNE, which may be related to the increase of NO content and the expression of VEGF mRNA.