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方法:免疫细胞化学ABC法;目的:调查N-甲基-D-天冬氨酸型受体、磷酸激活的谷氨酰氨酶和谷氨酸在大鼠三叉神经尾侧亚核内的分布及其匹配关系.结果:N-甲基-D-天冬氨酸型受体1和2A/B亚单位免疫阳性胞体和纤维密集分布于大鼠三叉神经尾侧亚核的浅层(Ⅰ、Ⅱ层),磷酸激活的谷氨酰氨酶和谷氨酸免疫阳性胞体分布于三叉神经尾侧亚核各层,尤以浅层密集.磷酸激活的谷氨酸氨酶和谷氨酸阳性纤维及终未样结构主要分布于三叉神经尾侧亚核浅层.以上结果表明三叉神经尾侧亚核内磷酸激活的谷氨酰氨酶和谷氨酸免疫阳性纤维及终末样结构的分布与N-甲基-D-天冬氨酸型受体阳性胞体和纤维的分布在总体上是相互匹配的.结论:三叉神经尾侧亚核内的谷氨酸可能通过作用于N-甲基-D-天冬氨酸型受体的不同亚单位而发挥多种生理功能.
Methods: Immunocytochemical ABC method; Objective: To investigate the distribution of N-methyl-D-aspartate receptor, phospho-activated glutaminase and glutamate in the subnuclei of the rat trigeminal nerve And their matching relationship.Results: Immunopositive cell bodies and fibers of N-methyl-D-aspartate receptor 1 and 2A / B subunits were densely distributed in the superficial layer of the subnucleus caudalis of rat trigeminal nerve (Ⅰ, Ⅱ), phospho-activated glutaminase and glutamate-immunoreactive cell bodies distributed in the subfractor nucleus of the trigeminal nerve, especially in the shallow layer.Phosphoramidate-activated glutamate aminotransferase and glutamate-positive fibers And the end-like structure is mainly distributed in the superficial layer of the trigeminal nerve subfractor tail.The above results show that the distribution of phospho-activated glutaminase and glutamate-immunoreactive fibers and terminal-like structure in the tail nucleus of the trigeminal nerve with The distribution of N-methyl-D-aspartate receptor-positive cell bodies and fibers were generally matched with each other.Conclusion: Glutamic acid in the caudal subnucleus of the trigeminal ganglion may act on N-methyl- D-aspartate receptor of different subunits and play a variety of physiological functions.