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为了研究细胞因子白细胞介素-1（IL-1）和白细胞介素-2（IL-2）对神经系统兴奋性的调节作用,将IL-1β和IL-2分别作用于培养的大鼠大脑皮质神经元,然后观察其对谷氨酸（Glu）和 γ-氨基丁酸（GABA）免疫反应神经元的影响,并对实验结果进行图像分析和统计学处理。结果显示:IL-1β（10~5u/L）作用于培养的神经元后,Glu免疫反应神经元增加（P<0.01）,但不引起GABA免疫反应神经元数目的变化;而 IL-2（10~4 u/L）处理后,使Glu免疫反应神经元增加（P<0.05）,使GABA免疫反应神经元急剧减少（P<0.01 ）。以上结果提示,IL-1β和IL-2可能通过影响脑内Glu和GABA的代谢过程而提高大脑皮质神经元的兴奋性,此研究结果也为免疫—神经—内分泌网络学说提供了形态学资料。
In order to investigate the regulatory effect of cytokines interleukin-1 (IL-1) and interleukin-2 (IL-2) on the nervous system excitability, IL-1β and IL-2 were applied respectively to cultured rat brain Cortical neurons, and then observe their effects on glutamate (Glu) and γ-aminobutyric acid (GABA) immunoreactive neurons, and the results of the image analysis and statistical analysis. The results showed that the number of Glu immunoreactive neurons increased (P <0.01) but not the number of GABA immunoreactive neurons when IL-1β (10-5u / L) 10 ~ 4 u / L) increased the number of GDNF immunoreactive neurons (P <0.01). These results suggest that IL-1β and IL-2 may enhance the excitability of neurons in the cerebral cortex by affecting the metabolic processes of Glu and GABA in the brain. The results of this study also provide morphological data for the immune-neuroendocrine network theory.