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标准化接种的玉米小斑病菌 T 小种尽可能用同一个时间内产生的分生孢子,干燥收集,贮藏在液态氮中。孢子按重量比悬浮在矿油中,用 aliguot inoculator(等份接种器)喷射植物。从玉米叶洋菜培养基,玉米叶片和滤纸上收集的孢子密封于口径为5毫米宽的玻璃瓶内,贮藏在一196℃低温的液态氮中,其余的干放在室温下。定量地对植物接种悬浮在 Soltrol 170(无植物毒素的矿油)中的孢子,接种植物距喷雾器60公分下,以每分钟20转旋转,喷雾9秒钟,当油挥发后,将植物放在保湿箱内。试验结果:孢子在液态氮中贮藏一年,
The standardized inoculum of T. solani T was collected and stored in liquid nitrogen as much as possible with conidiospores produced in the same time. Spores are suspended in mineral oil by weight, and the plants are sprayed with aliguot inoculator. Spores collected from maize leaf culture medium, maize leaves and filter paper were sealed in glass vials measuring 5 mm wide, stored at a low temperature of 196 ° C in liquid nitrogen, and the rest dried at room temperature. The plants were inoculated quantitatively with the spores suspended in Soltrol 170 (phytotoxin-free mineral oil) and the inoculated plants were spun at 20 rpm for 60 seconds from the nebulizer and sprayed for 9 seconds. After the oil had volatilized, the plants were placed on Moisturizing box. Test Results: Spores were stored in liquid nitrogen for one year,