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杂交瘤细胞的长期保存,一般都是冻存在液氮(或氮气)中,所用培基中含有胎牛血清(FCS)及二甲基亚砜(DMSO)。这种冻存杂交瘤细胞方法虽安全可靠,但需要液氮装置,并经常补充液氮,价值甚高。本文作者设计了一种以牛奶衍生物代替FCS作为冻存细胞的培基,置-80℃冰箱长期保存细胞的方法。牛奶衍生物为新鲜牛奶经过67℃加热处理,离心去脂肪、去细胞碎片的浓缩蛋白液。为了作对比,配制两种细胞冻存液,培基A含有40％牛奶衍生物和10％DMSO,培基B含有40％FCS及10％DMSO。实验用细胞是分泌抗人促性腺激素
The long-term storage of hybridoma cells is generally frozen in liquid nitrogen (or nitrogen) with fetal calf serum (FCS) and dimethyl sulfoxide (DMSO). Although this method of cryo-hybridization of tumor cells is safe and reliable, it requires liquid nitrogen devices and frequently replenishes liquid nitrogen, which is of great value. The authors designed a method that uses milk derivatives instead of FCS as the culture medium for cryopreservation cells and stores cells in a -80 ℃ refrigerator for a long time. Milk derivatives are fresh milk, heat-treated at 67 ° C, centrifuged to remove fat, cell debris, concentrated protein solution. For comparison, two cell cryopreservation solutions were prepared. Peracetamol A contained 40% milk derivative and 10% DMSO, and Pemetrexed B contained 40% FCS and 10% DMSO. Experimental cells are secreting anti-human gonadotropins