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以采自山西省沁水县历山等8处植物多样性丰富的山区10个华北耧斗菜材料和5个耧斗菜园艺品种为试材,采用SRAP(sequence-related amplified polymorphism)分子标记技术进行了亲缘关系分析。结果表明:从88对引物中筛选出17对多态性丰富的引物组合用于PCR扩增,得到扩增条带352条,其中多态性条带307条,多态性比率为87.2%。利用NTSYSpc-2.0软件进行UPGMA聚类分析,分别得出15个供试材料与10个华北耧斗菜材料间的亲缘关系树状图。15个供试材料的相似系数为0.6416~0.8571,聚类结果遵循植物学分类。10个华北耧斗菜材料的相似系数为0.7218~0.8571,生境是影响华北耧斗菜聚类结果的主要因素,地理分布为次要因素。
A total of 10 North China buckets of cabbage and 5 hors d’oeuvres horticultural cultivars collected from 8 plant diversity-rich mountainous areas in Qishui County, Shanxi Province were used as test materials and sequenced by SRAP (sequence-related amplified polymorphism) Conducted a kinship analysis. The results showed that 17 pairs of primers were selected from 88 pairs of primers for PCR amplification. There were 352 amplified bands, of which 307 were polymorphic. The polymorphic ratio was 87.2%. The UPGMA cluster analysis was conducted by using NTSYSpc-2.0 software, and the dendrograms of the relationship between 15 tested materials and 10 Chinese cabbage materials were obtained respectively. The similarity coefficients of 15 tested materials ranged from 0.6416 to 0.8571. The clustering results followed the botanical classification. The similarity coefficient of 10 Chinese cabbages was 0.7218 ~ 0.8571. Habitat was the main factor affecting the clustering results of Cabernet Sauvignon in North China. The geographical distribution was the secondary factor.