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目的通过用酶联免疫印迹技术检测353例自身免疫性结缔组织病患者血清中八种ENA多肽抗体,探讨ENA多肽抗体的检测在自身免疫性疾病诊断中的应用价值。方法采用免疫印迹技术对自身免疫性疾病患者及正常人对照血清做ENA多肽抗体检测。结果各种自身免疫性疾病如SLE、MCTD、SS、PSS、PM/DM及PA等疾病的八种ENA抗体阳性率均明显有不同程度升高:70例正常人对照组几乎未检测到ENA抗体。结论用免疫印迹技术检测ENA多肽抗体,快速、敏感、特异性强,有助于多种系统性自身免疫性疾病的诊断分型和鉴别诊断,有助于不同风湿病患者的早期诊断及时治疗及预后判断。
OBJECTIVE: To detect eight ENA polypeptide antibodies in serum of 353 patients with autoimmune connective tissue disease by enzyme-linked immunosorbent assay (ELISA), and to explore the value of ENA antibody in the diagnosis of autoimmune diseases. Methods Immunoblotting was used to detect ENA peptide antibodies in patients with autoimmune diseases and normal controls. Results The positive rates of eight ENA antibodies in various autoimmune diseases such as SLE, MCTD, SS, PSS, PM / DM and PA were significantly increased in different degrees: almost no ENA antibody was detected in 70 normal controls . Conclusion The detection of ENA polypeptide antibody by immunoblotting is fast, sensitive and specific. It is helpful for the diagnosis and differential diagnosis of various systemic autoimmune diseases. It is helpful for the early diagnosis and timely treatment of patients with different rheumatic diseases and Prognosis.