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目的探讨微波辐射对大鼠大脑皮层和海马突触囊泡相关蛋白如突触素I(synapsinI),囊泡相关膜蛋白-2(vesicle-associated membrane protein-2,VAMP-2)和突触融合蛋白(syntaxin)以及突触囊泡蛋白(synaptophysin)表达的影响。方法采用30mW/cm2微波辐射Wistar雄性大鼠,于辐射后6h,1,3和7d取材,通过免疫蛋白印迹检测大脑皮层和海马突触体突触素I,VAMP-2,突触融合蛋白和突触囊泡蛋白表达的改变;采用免疫共沉淀检测VAMP-2和突触融合蛋白相互作用的改变。结果30mW/cm2微波辐射后皮层突触素I于辐射后3d表达减少(P<0.01);海马突触素I表达1d增加,3d减少,7d又增加的波动(P<0.01)。皮层和海马突触囊泡蛋白于辐射后7d表达增加(P<0.01),VAMP-2和突触融合蛋白于辐射后7d内表达均降低。VAMP-2和突触融合蛋白相互作用在皮层于辐射后3d减弱,而海马于辐射后7d内明显减弱(P<0.01)。结论微波辐射可引起大脑皮层和海马突触囊泡蛋白表达异常,进而影响突触传递功能。
Objective To investigate the effects of microwave irradiation on the synaptic vesicle-related proteins such as synapsin I, VAMP-2 and synaptic fusion in rat cerebral cortex and hippocampus Syntaxin and synaptophysin expression. Methods Wistar rats were irradiated with microwaves at 30mW / cm2 and harvested at 6h, 1, 3 and 7d after irradiation. The concentrations of synaptophysin I, VAMP-2, synaptophysin and synaptophysin in cerebral cortex and hippocampus were detected by immunoblotting Synapse vesicle protein expression changes; co-immunoprecipitation detected VAMP-2 and synaptic fusion protein interaction changes. Results After 30mW / cm2 microwave irradiation, the expression of synaptophysin I in the cortex decreased 3 days after radiation (P <0.01). The expression of synaptophysin I in the hippocampus increased 1d, 3d and 7d respectively (P <0.01). The expression of synaptophysin in the cortex and hippocampus increased 7 d after radiation (P <0.01). The expression of VAMP-2 and synaptotagmin decreased at 7 d after irradiation. The interaction between VAMP-2 and synaptotagmin was weakened in the cortex 3 days after irradiation, while the hippocampus was significantly weakened within 7 days after irradiation (P <0.01). Conclusion Microwave radiation can cause synaptic vesicle protein abnormalities in the cerebral cortex and hippocampus, which may affect synaptic transmission.