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目的了解贵阳市市售水产品霍乱弧菌污染状况,为制订预防控制霍乱的措施提供依据。方法 2008-2010年采集贵阳市水产品批发市场甲鱼、牛蛙及其养殖水标本,培养分离霍乱弧菌和鉴定菌株,对检出的霍乱弧菌采用PCR方法进行CT毒力基因检测。结果从234份水产品标本中检出霍乱弧菌20株,检出率8.55%;O1群18株(90%),小川型8株,稻叶型10株,O139群2株(10%);其中甲鱼检出率3.37%(3/89),牛蛙检出率17.05%(15/88),养殖水检出率3.51%(2/57)。牛蛙与甲鱼、养殖水检出率差异有统计学意义(χ2=13.03,P<0.01),牛蛙中霍乱弧菌检出率较高。2010年与2008年、2009年检出率差异有统计学意义(χ2=10.14,P<0.01),2010年霍乱弧菌检出率较高。20株霍乱弧菌均未检出CT毒力基因。结论贵阳市水产品中存在霍乱弧菌污染,以O1群为主,均为非产毒株,需继续加强水产品中霍乱弧菌的监测,及时发现传染源,以便积极防控。
Objective To understand the status of Vibrio cholerae polluting aquatic products in Guiyang and to provide evidence for the prevention and control of cholera. Methods The specimens of turtle, bullfrog and cultured bullfrog were collected from the aquatic product wholesale market in Guiyang from 2008 to 2010. Vibrio cholerae were isolated and identified. The CT virulence genes of Vibrio cholerae were detected by PCR. Results 20 strains of Vibrio cholera were detected in 234 samples of aquatic products, with a detection rate of 8.55%. 18 strains (90%) of O1 group, 8 strains of Ogawa, 10 strains of rice leaf and 2 strains of O139 (10% ; The detection rate of turtle was 3.37% (3/89), the detection rate of bullfrog was 17.05% (15/88), and the detection rate of breeding water was 3.51% (2/57). There was a significant difference in the detection rates of bullfrog and soft-shelled turtle (χ2 = 13.03, P <0.01), while the prevalence of V. cholera in bullfrog was higher. In 2010 and 2008, the detection rate in 2009 was significantly different (χ2 = 10.14, P <0.01). In 2010, the detection rate of Vibrio cholerae was higher. None of the 20 V. cholerae strains detected CT virulence genes. Conclusion There is Vibrio cholerae contamination in aquatic products in Guiyang City. Most of them are O1 group, all of them are non-virus-producing strains. Vibrio cholerae in aquatic products should be continuously monitored for the detection of Vibrio cholerae in time so that they can be actively controlled.