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为探讨冠状血管内皮细胞在氧自由基致心肌损伤中的作用，采用电解法（恒流，5mA，2min）产生氧自由基（OFR），观察OFR对保留冠状血管内皮及去冠状血管内皮的Langendorff大鼠心脏心率（HR）、心律、冠脉灌注压（CPP）、左室内压峰值（LVSP）及左室舒张末压（LVEDP）、冠脉流出液中乳酸脱氢酶（LDH）含量、心肌组织中两二醛（MDA）及Na+－K+ATPase水平的影响。结果：去内皮组CPP、LVEDP、LDH、MDA及心律失常严重指数（ASI）均显著低于冠脉内皮保留组（P＜0．05或P＜0．01），而Na+－K+ATPase显著高于冠脉内应保留组（P＜0．05）。提示：去冠脉内皮细胞可减轻OFR对心肌的损伤，冠脉内皮至少部分介导OFR对心肌的损伤，亦说明冠脉内皮细胞在心肌损伤中所发挥的重要作用。
In order to investigate the role of coronary endothelial cells in oxygen free radical induced myocardial injury, oxygen free radicals (OFR) were generated by electrolysis (5mA, 2min), and observed the effect of OFR on the Langendorff retention of coronary endothelium and endothelium Heart rate (HR), heart rate, coronary perfusion pressure (CPP), left ventricular pressure peak (LVSP) and left ventricular end diastolic pressure (LVEDP), coronary lactate dehydrogenase (LDH) The effect of MDA and Na + -K + ATPase in the tissue. Results: Serum CPP, LVEDP, LDH, MDA and ASI in the endothelium group were significantly lower than those in the endothelium-preserved coronary artery group (P <0.05 or P <0.01), while the levels of Na + -K + ATPase Higher than the coronary artery should be retained group (P <0.05). Tip: to coronary endothelial cells can reduce OFR damage to the heart, atherosclerotic heart endothelial OFR at least partially mediated damage, also shows that coronary endothelial cells play an important role in myocardial injury.