R-HPLC测定人体血清及尿液中头孢氨苄浓度

• 作 者: 陈桂良 王登明 郑颉
• 机 构: 上海医科大学药剂教研室,上海医科大学药剂教研室,上海医科大学药剂教研室 上海 200032 1990届硕士研究生 上海市药品检验所,上海 200032,上海 200032
• 出 处: 现代应用药学
• 发表日期: 1991年02期
• 关键词: 人体血清 R-HPLC测定 头孢氨苄 药物动力学参数 血清蛋白 稀释法 线性范围 氯化铵溶液 高效液相测定 实验条件

本文应用沉淀血清蛋白及尿样直接稀释法,使用YWG-C_(18)键合固定相,以甲醇:0.01M氯化铵溶液:冰醋酸(15:85:0.015V/V/V)为流动相系统,建立了人体血清及尿样中头抱氨苄的反相高效液相测定方法。本法精确度高,血清和尿样中的平均天内天间变异系数分别为1.50％,1.03％和1.72％,1.32％。检测波长为254nm,最低检测浓度血清中为0.5μg/ml,尿液中为2.5μg/ml,血清中线性范围2.5—40μg/ml,尿液中为10—500μg/ml,分析周期为12分钟,本法快速、灵敏。

In this paper, precipitated serum albumin and urine samples were directly diluted with YWG-C 18 bonded stationary phase and methanol: 0.01 M ammonium chloride solution (15:85:15 V / V / V) Phase system, the method of reversed-phase high performance liquid phase determination of huperin in human serum and urine was established. The accuracy of this method is high, and the mean intra-day coefficient of variation in serum and urine samples are 1.50%, 1.03% and 1.72%, 1.32% respectively. The detection wavelength was 254nm. The lowest detection concentration was 0.5μg / ml in serum, 2.5μg / ml in urine, 2.5-40μg / ml in serum, 10-500μg / ml in urine, and the analysis period was 12 minutes This law is fast and sensitive.