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目的探讨丙烯腈(acrylonitrile,ACN)对雄性小鼠生殖细胞毒性作用机制。方法将50只成年健康SPF级昆明种雄性小鼠,按体重随机分成阴性对照组(生理盐水0.01 ml/g)、ACN1.25、2.50和5.00 mg/kg组,连续腹腔注射5 d,1次/d;阳性对照组腹腔注射环磷酰胺(40 mg/kg)1次。于首日染毒后的第35天处死小鼠,取材,检测分析。结果 ACN各剂量组间小鼠血清FSH、ICSH、T变化差异无统计学意义;生殖细胞周期以及睾丸组织LDH、ACP和AKP活力,ACN各剂量组间差异无统计学意义;ACN 2.50和5.00 mg/kg组的MDA含量较阴性对照组显著升高(P<0.05);ACN 1.25 mg/kg组SOD活性与阴性对照组比较明显降低(P<0.05)。结论本实验条件下ACN对血清性激素、细胞周期及睾丸组织标志酶活力影响不大;ACN可能会引起脂质过氧化损伤,降低机体清除ROS的能力。
Objective To investigate the mechanism of germ cell cytotoxicity induced by acrylonitrile (ACN) in male mice. Methods Fifty adult healthy SPF Kunming male mice were randomly divided into negative control group (saline 0.01 ml / g), ACN 1.25, 2.50 and 5.00 mg / kg groups, / d; positive control group intraperitoneal injection of cyclophosphamide (40 mg / kg) 1 time. The mice were sacrificed on the 35th day after the first day of exposure and the materials were collected and analyzed. Results There was no significant difference in serum FSH, ICSH and T between the different doses of ACN. There was no significant difference in the germ cell cycle and the activities of LDH, ACP and AKP between testis and ACN groups. The ACN 2.50 and 5.00 mg (P <0.05). The activity of SOD in ACN 1.25 mg / kg group was significantly lower than that in the negative control group (P <0.05). Conclusion ACN has little effect on serum sex hormones, cell cycle and testicular tissue enzyme activity under the experimental conditions. ACN may cause lipid peroxidation damage and reduce the ability of the body to scavenge ROS.